|MadSci Network: Molecular Biology|
When you sequence DNA using the Sanger Method, each of the four test tubes contains a complete set of nucleotides (plus a specific ddnucleotide). Since each test tube has all the normal nucleotides, the longest strand (which will show up in the electrophoresis as the first base), should show up in every column of the electrophoresis. Thus, how do you determine which base is the first one?
Re: When using the Sanger Method, how is the first base identified?
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