MadSci Network: Biochemistry
Query:

Subject: how lineweaver burk plots(1/V vs. 1/[S]]determine kind of enzyme inhibition

Date: Sun Jul 13 19:56:03 1997
Posted by Chris G. Stavitzke
Grade level: 10-12
School: Lake Central High School
City: St. John State/Province: IN
Country: Lake
Area of science: Biochemistry
ID: 868841763.Bc
Message:
My name is chris and I'm involved in a research project down at IU in 
Bloomington.  I'm working with an enzyme kinetics project to determine
whether the inhibition of enzymatic activity is competitive or 
noncompetitive.  I am using the enzyme horseradish peroxidase and the
substrate o-phenylenediamine (OPD).  I am using a colorimeter to help
me graph time vs. absorbance. I use this information for my plots.   
For my lineweaver burk plot, I have one line plotted with just 
different substrate concentrations, another line with 1 mL of Ni(NO3)2 
inhibitor and another line with 2 mL of this inhibitor.  I cannot 
figure out how this plot (where the three lines intersect) helps me to 
determine whether the inhibition is competitive or noncompetitive.

Re: how lineweaver burk plots(1/V vs. 1/[S]]determine kind of enzyme inhibition

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