|MadSci Network: Biochemistry|
My name is chris and I'm involved in a research project down at IU in Bloomington. I'm working with an enzyme kinetics project to determine whether the inhibition of enzymatic activity is competitive or noncompetitive. I am using the enzyme horseradish peroxidase and the substrate o-phenylenediamine (OPD). I am using a colorimeter to help me graph time vs. absorbance. I use this information for my plots. For my lineweaver burk plot, I have one line plotted with just different substrate concentrations, another line with 1 mL of Ni(NO3)2 inhibitor and another line with 2 mL of this inhibitor. I cannot figure out how this plot (where the three lines intersect) helps me to determine whether the inhibition is competitive or noncompetitive.
Re: how lineweaver burk plots(1/V vs. 1/[S]]determine kind of enzyme inhibition
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