MadSci Network: Microbiology |
Water molecules are composed of 2 atoms of hydrogen (H) and 1 atom of Oxygen (O). It is commonly written as H2O or HOH. However, water and most other liquids really exist in the form of positive and negative ions. In the case of water H+ and OH-. pH is a measure of this hydrogen ion concentration in a solution. The higher the H+ concentration (or lower the pH) the more acidic a solution. As the H+ concentration is lowered (as the pH goes up) the solution becomes more basis or alkaline. (EX: Vinegar would be a mildly acidic solution and bleach would be a highly basic solution.) The pH scale goes from 1-14, with 1 being highly acidic and 14 being highly basic. A pH of 7.0 would be considered neutral or not acidic or basic. Almost all living organisms and microorganisms live best in solutions close to this neutral pH (7.0). Their internal machinery or enzyme systems tend to operate best at these neutral conditions. If you add an acid to water you will lower the pH and the addition of a base such as bleach will raise the pH. As a solution moves away from neutrality, pH 7.0, changes occur in the internal operation of the cells and eventually the cell machinery will shutdown, the cells will rupture and die. The solutions you are adding can also have other effects unrelated to pH that may cause direct toxicity to the microorganisms. These toxic effects could directly shutdown cell functions leading to death. therfore, your solutions could have multiple effects on the microorganisms. Additional information on the effects of pH on microorganisms should be found in basis Environmental Sciences or Environmental Microbiology texts in your local Library.
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