|MadSci Network: Genetics|
Dear Sir or Madam Thanks very much for your service. Here's my question: Wouldn't it be a good idea to use the common protein domains for getting a coarse overview about the human (or other species') genome within a short time? There are about 150 proteine parts that occur again and again in the proteines of the living cell. Recognizing them in the amino acid sequence of a newly sequenced proteine (corresponding to a newly sequenced gene) helps very much in understanding the tertiary structure of the proteine and it's function, since common domains indicate a common origin of the gene (mostly by recombination of other genes or the process of gene doubling and subsequent specialization) and there are certain domains always responsible for the same purpose (for example the inner side of membrane proteines). Of course, there are little mutations (point mutations) in the instances of a domain, but knowledge about the occurences of all the domains within the human genome would help in any case. So one could use the domains (I mean the corresponding DNA) as DNA probes (marked by radioactive isotopes or by fluorescence) in order to get the overview over the relevant parts of the genome. One could also use such domain-DNA probes for rapidly investigating the expression pattern of the domains in specialized cells by using them on the cell's mRNA. Thank you for your time. Best regards Bruno mailto:email@example.com
Re: Using common proteine domains for a faster Human Genome Project?
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