MadSci Network: Botany |
Ian, The first thing you need to do is a little research in order to develop a good hypothesis. Look for information on plant photosynthesis and light wavelengths. You should have in mind what wavelengths plants use and what wavelengths each of your treatments provides; then you will be able to make a good hypothesis based on the information you find. It sounds like you're on the right track with the experiment itself. You will need to think about what kind of plant you want to use. I would look for something that has a short life cycle (if you use garden seeds, the packet will indicate this as "days to harvest") and that will be easy to measure (not a vine, like pole beans or peas, or a rosette, like radish or cabbage). A few controls that I would suggest are: 1. Make sure that all factors other than light are the same for all your plants (try to keep the temperature the same, keep them all well watered, etc.) 2. Keep the duration of light the same for the sunlight and black light treatments; i.e., turn your black light on at sunrise and off at sunset. That way you know that any differences you observe are due only to wavelength and not to how long the plants received light. You should also make sure that you have several replications within each treatment. That is, grow 5 or 10 (or as many as you have room for) plants in sunlight, and the same number in black light and in the dark. When you are done with the experiment, you can average the results of all the plants in each treatment and compare the averages. Just like us, plants have genetic variability; some individual plants will grow better than others just because their genes are different. If you only have one plant in each treatment, you can't be sure that the differences you see aren't just individual differences in the plants' genes. The more plants you use, the more confidence you can have that your results aren't due to these individual differences. As you are setting up, think about what kinds of measurements you will be taking, how often, and for how long. How are you going to determine "good" versus "bad" responses? Survival for some period of time? Leaf size? Leaf color? Number of leaves? Plant height? Development of flowers and fruit? Again, a little research will help you determine how you should judge your plants' growth. Some plant responses to adverse conditions will make measurements like height unreliable by themselves (hint: look up etiolation), so you should consider using a group of characteristics to describe the condition of your plants. Good luck, and have fun! Brantlee Spakes Department of Plant Biology Arizona State University Box 871601 Tempe, AZ 85287-1601
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