MadSci Network: Biochemistry |
The question is much simpler than the answer to give. The first question should be WHAT do you want to measure and then how? When I assume that you want to use ultraviolet spectroscopy [UV] to measure the protein content of casein and BSA in solution then you have the following possibilities. First of all: all proteins including the caseins, (which is actually a group of at least 4 different ones [alpha s1, alpha s2, beta and kappa-casein]) are composed of amino acids linked together through the peptide bond. A characteristic feature of the peptide bond is UV absorption between 200 and 230 nm. It is very common to measure proteins using UV-detection at 214 nm. Furthermore most, but not all, proteins contain aromatic amino acid residues like Tyrosine and Tryptophan. These amino acids are responsible for UV-absorption in the 260-280 nm range therefore, apart from using detection at 214, proteins are measured at 280 nm. As a rule of thumb [so it is dangerous and different for each protein] it is said that 1mg/ml has a UV-absorption of 1 Unit at 280 nm. What to choose? You need to make a series of standard protein solutions and measure them at e.g. 280 nm and use that for you sample. Remember to state your results 'expressed' as mg reference protein, either BSA or the casein [mix]. If this is not clear enough repeat your question more defined or mail me at pdvwasse@caiw.nl
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