MadSci Network: General Biology |
Noelene: This what I do all the time for growing ciliates, amoeba, all sorts of flagellates (the smallest of the protists) and sometimes even heliozoans: Collect water from a pond, puddle or stream, and place in a jar. Add uncooked rice (a dozen grains is fine), and wait a few days. (Keep it away from direct sunlight, and cover with plastic so it doesnt dry out.) The rice grains supplies starch which feeds the bacteria that will feed the protists. Sometimes all you see at first are tiny flagellates, but if you wait a few days some bigger ciliates will become abundant. Protists are also common in damp soil, so you may want to add some to the jar. If you can get someone to collect a jar of "activated sludge" from the aeration basin at your local sewage treatment plant (if you have one nearby) it will be full of protists already, especially stalked ciliates including Vorticella that are easy to watch, since they do not swim. Finally, if you luck out and find some termites, which can be found in a tree stump sometimes, their abdomens are full of the most remarkable protists: To see them, cut off the termite's "rear end" and add a drop of dilute salt solution (.7% by weight). (Don't let any of the termites get loose.) Squash this abdoment in the drop underneath a cover slip that has been coated with vasoline along the edge (do this by spreading a thin coat of vasoline on your palm, then gently scape the coverslide (all four edges) on your palm, being careful not to get fingerprints on it. This prevents oxygen from rapidly killing the protists. Then enjoy the show. Good luck! Dean Jacobson, plankton and protist watcher
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