| MadSci Network: Biochemistry |
Hopefully, the following is of use - they are drawn from Asimov's Chronology of Science and Technology: Nucleic Acid (1869) The classification of foodstuffs into carbohydrates, fats, and proteins seemed to be satisfactory for the early part of the 19th Century. However, in 1869, a Swiss biochemist, Johann Friedrich Miescher (1844 - 1895) was able to isolate a substance from the remnants of cells in pus that did not belong to any of these three classes. It contained carbon but also had both nitrogen and phosphorus. Miescher took his new discover to fellow biochemist, Felix Hoppe-Seyler who independently investigated the matter. When he discovered a similar substance in yeast, the discovery was announced. Since the material seemed to originate in cell nuclei, it was called "nuclein" at first, and later, because of its acidic properties, the name was changed to "nucleic acid". That is, Miescher and Hoppe-Seyler had isolated and characterized a fragment of a cell that appeared to be associated with the nucleus, appeared to be an acid, and appeared to not be any of the previously identified cellular fragments. Purines and Pyrimidines (1885) Since the nucleic acids had been discovered by Miescher, little had been done to determine their molecular structure. The German biochemist, Albrecht Kossel (1853-1927) took up the challenge. He got rid of the proteins associated with the nucleic acids and worked on the material itself. By 1885, he had obtained substances from it that included the double- ring purines and single-ring pyrimidines. He isolated two purines, adenine and guanine and three different pyrimidines, uracil, cytosine, and thymine. He also decided that a sugar molecule was present but couldn't tell which one. So, by 1885, the nucleic bases were identified. However, because Kossel was working with whole cell extracts, he did not distinguish the source of these bases as either RNA or DNA - simply the constituents of nucleic acid. Ribose (1909) In 1909, the Russian-born chemist Phoebus Aaron Theodor Levene (1869 - 1940) extracted a sugar from nucleic acid and identified it as "ribose". It is a five carbon sugar, forming a pentagonal ring. Not all of the nucleic acid molecules possessed it, but those that did came to be known as "ribose nucleic acid" - abbreviated RNA. Thus, the name "RNA" comes from a literal description of the content of the material. It is a series of nucleic bases linked to a sugar unit (sugar units are linked as phosphate esters). DNA or deoxyribose nucleic acid was the other form or "not ribose" form of nucleic acid and it wasn't isolated until 1929. It wasn't until the mid-1950's, with the discovery of the double helix by Watson and Crick, that the importance of DNA and RNA to life were discovered. In essence, DNA is the recipe for all of the proteins and other molecules that make up our bodies. RNA is the chef who reads the recipe and makes the compounds. That is a crude analogy for what RNA is and does, because there are three forms of RNA and they all play different roles, but the purpose of RNA is to take genetic information from DNA and translate it into proteins, which subsequently fold-up to become enzymes, which make all of the compounds from which we are formed. Hope this helps.
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