MadSci Network: Molecular Biology

Subject: How does the restriction enzyme know where to cut the DNA strand?

Date: Mon Apr 19 20:19:38 1999
Posted by Kimberly Won
Grade level: 10-12 School: John F. Kennedy
City: Sacramento State/Province: California Country: United States
Area of science: Molecular Biology
ID: 924571178.Mb

I am currently taking molecular biology as a freshman at John F, Kennedy.  Our 
class has just finished studying about recombinant DNA and how they are formed. 
We learned that recombinant DNA is a DNA molecule made of two or more different 
DNA molecules.  There are different DNA restriction sites, which are made of 
specific nucleotides.  We know that the restriction enzymes are the enzymes that 
cut these specific sequences to create sticky and blunt ends.  However, I are 
interested how the restriction enzymes actually know where to cut the DNA 

Thank you very much for your time.  If you could write a response to my 
question, I would greatly appreciate it.  Thank you!

Re: How does the restriction enzyme know where to cut the DNA strand?

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