MadSci Network: Molecular Biology

Subject: How do ligases 'tape' the sticky ends of DNA molecules?

Date: Wed Apr 14 10:24:49 1999
Posted by Brandon Gin
Grade level: 10-12 School: JOhn F. Kennedy
City: Sacramento State/Province: CA Country: United States
Area of science: Molecular Biology
ID: 924103489.Mb

How do ligases "tape" the sticky ends of DNA molecules?  How do restriciton 
enzymes cut the DNA molecules?
Dear Scientist,
We are high shcool students that have decided to ask you about the details in 
puttin together recombinant DNA.  We have learned that restriction enzymes cut 
DnA molecules and that ligases "tape" the sticky ends together.  However, we are 
wondering how these molecules do these things.  Is anything else involved in 
cutting or taping DNA molecules?  If so, could you please tell us what they are 
and why they're needed?  Thank you for your time.  I hope that you decide to 
answer our questions.  Thank you.
Brandon Gin
Gina Kim
Karen Leung

Re: How do ligases 'tape' the sticky ends of DNA molecules?

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