MadSci Network: Biochemistry
Query:

Subject: Why glycerol reduces the amount of ice in cells during cryopreservation?

Date: Tue Apr 20 14:22:25 1999
Posted by Tina Julson
Grade level: teacher/prof School: University of Sioux Falls
City: Sioux Falls State/Province: South Dakota Country: USA
Area of science: Biochemistry
ID: 924636145.Bc
Message:

Glycerol is a cryoprotective when freezing  sperm cells in liquid 
nitrogen. 
Without the cryoprotection of glycerol, cell death might occur. 
However, if glycerol is present there is a reduction in the 
amount of ice formed at subzero tempatures.  I was wondering: 
What is it about glycerol that reduce the amount of ice during 
freezing?
 Also, glycerol also reduces the build up in salt concentration 
in the cell? Could you help describe How/Why glycerol reduces the 
solution effects (salt concentration)?



Re: Why glycerol reduces the amount of ice in cells during cryopreservation?

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