| MadSci Network: General Biology |
That is a great research project you started to do. Regarding the amount of creatine, you already did the right thing. You did research on it and got some approximate ideas about how much they might be able to survive. The exact figures are unknow - that is science. If someone would have done exactly what you want to know, this would not be real science anymore, but just repetition of what is already known. No animal should go through experiments just for this, not even Drosophila. Every scientist - in this case you - is responsible for the animals involved. As I (and others on our network we contacted) can not provide exact figures, the only way out that I see: you have to test a whole range of concentrations for the effects on Drosophila. If you have a really good idea you can do a series with 0% 20% 40% 60% 80% and 100% of your maximum concentration. Hopfully you can identify graded responses to your treatments. If you have no idea about the concentrations to use, start an experiment on the logarithmic scale. Use the maximum concentration reasonable, as well as one tenth, one hundereth, on thousandth, one thenthousandth of it. This covers much more concentration difference than the first set of experiments, but you might end up with no effects in one concentration and all dead in the next higher concentration. In principle I am happy to discuss any of your ideas and experiments, how you want to analyce the data and how to present them. If you want this you can email me jzies001@umaryland.edu. Hope that helps Dr. Jurgen Ziesmann
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