MadSci Network: General Biology
Query:

Re: Will creatine have any affect on the fatality of drosophila?

Date: Fri Nov 5 09:57:39 1999
Posted By: Jurgen Ziesmann, MadSci Admin
Area of science: General Biology
ID: 940345954.Gb
Message:

That is a great research project you started to do.

Regarding the amount of creatine, you already did the right thing. You did 
research on it and got some approximate ideas about how much they might be able 
to survive.
The exact figures are unknow - that is science.
If someone would have done exactly what you want to know, this would not be real 
science anymore, but just repetition of what is already known. No animal should 
go through experiments just for this, not even Drosophila. Every scientist - in 
this case you - is responsible for the animals involved. 

As I (and others on our network we contacted) can not provide exact figures, 
the only way out that I see: you have to test a whole range of concentrations 
for the effects on Drosophila.
If you have a really good idea you can do a series with 0% 20% 40% 60% 80% and 
100% of your maximum concentration. Hopfully you can identify graded responses 
to your treatments.
If you have no idea about the concentrations to use, start an experiment on the 
logarithmic scale. Use the maximum concentration reasonable, as well as one 
tenth, one hundereth, on thousandth, one thenthousandth of it. This covers much 
more concentration difference than the first set of experiments, but you might 
end up with no effects in one concentration and all dead in the next higher 
concentration.

In principle I am happy to discuss any of your ideas and experiments, how you 
want to analyce the data and how to present them.
If you want this you can email me jzies001@umaryland.edu.

Hope that helps
Dr. Jurgen Ziesmann




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