Re: How do you use glucose oxidase and peroxidase in the analysis of glucose?

Dear Gavin,

Glucose oxidase and peroxidase are used to detect glucose by what is known 
as a colorimetric assay. The assay works by coupling the activities of the 
two enzymes together. In the first step, glucose oxidase catalyzes the 
reaction:

glucose + oxygen -> gluconolactone + hydrogen peroxide

In the second step, the hydrogen peroxide is used by the peroxidase to 
convert a chemical substrate (called a chromogen) from an uncolored form 
into a colored one. In clinistix, the enzymes and the chromogen are 
attached to the stick. When glucose is present, the reactions described 
above take place, and the stick changes color. The principle was first 
described by Keston in 1956. (Keston, A., Specific colorimetric enzymic 
reagents for glucose. Anstracts papers, 129th Mtg. American Chemical 
Society, 31c).