MadSci Network: Molecular Biology

Re: What is the purpose of chromosome walking?

Date: Mon Dec 6 13:09:41 1999
Posted By: Christopher Carlson, Grad student Genetics
Area of science: Molecular Biology
ID: 944432152.Mb

Hi h5nl,

Your question is an important one, and one which makes those of us who have been in the field of genomics for even five years feel old. Chromosome walking is a technology, and as such it is about to become obsolete. Its main purpose was to allow scientists access to physical clones spanning a region of genetic interest. From physical clones it is possible to identify candidate genes, and thereby identify the precise molecular lesion causing a disease. In the post genome era, chromosome walking will be obsolete because we will simply dial up the databases and request the sequence between two markers without any benchwork at all.

To put this in historical perspective, the first major use of chromosome walking was to find the Cystic fibrosis gene, all the way back in 1989. At that time they had narrowed the region containing the gene to an interval between two markers on chromosome 7, but the sequence in between was not available. Therefore physical clones were isolated walking across the locus, used for candidate gene discovery, and ultimately lead to identification of the CFTR gene.

This process (genetic mapping followed by physical mapping followed by candidate gene analysis) became known as positional cloning, which has been wildly successful in isolating mutations causing many rare mendelian disorders, including the breast cancer genes BRCA1 and BRCA2 However, positional cloning is rapidly becoming obsolete, because almost every Mendelian disease with clear genetic loci has been positionally cloned, providing that more than two or three families can be found globally.

Anyhow, the short answer to your question is that chromosome walking was used to identify the surrounding sequence of a particular "unknown" gene which was known to lie between two markers.

Chris Carlson

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