|MadSci Network: Immunology|
In our lab, about the evolution of protein serums, we had 3 parts. The first part we're pretty sure we have figured out. We just took 6 serums of animals (donkey, goat, sheep, chicken, cow, and horse), electrophorese them, stain and destain. Simple enough. In the 2nd part, we put agar in a petri dish and made 7 wells, 6 in a circle, one in the middle. Cow antibodies went in the middle, and serums in the others. We were supposed to see white precipitate lines after incubation. What do the white precipitate lines show? Are the serums moving towards the antibodies vice versa or neither?We were trying to research the process but we can't find much. Then in part 3, we made 2 gels, putting serums in every other well, and electrophoresed, and put strips of paper w/ antibodies on them and incubated them. There are supposed to be white precipitate lines,but we didn't get any. We still have to know what was supposed to happen and what we did wrong. We need to be pointed in the right direction. Also, if you would have any suggestions as to where we could find a little bit more information on this if you can't help us out. Thank you so much! Nikki Little North Farmington High School
Re: What do white precipitate lines after you immunoelectrophorese show?
Try the links in the MadSci Library for more information on Immunology.