MadSci Network: Molecular Biology

Subject: How do Western blots work?

Date: Tue Nov 30 18:54:31 1999
Posted by Sanjiv Shah
Grade level: undergrad School: University of Vermont
City: Burlington State/Province: VT Country: USA
Area of science: Molecular Biology
ID: 944009671.Mb

I am interested in how the components of the Western Blots work. 

For eg. How does the stacker gel work? What does the acrylamide do? What 
role does the TEMED, ammonium persulfate, water-saturated n-butanol and 
Tris-HCL play in the SDS-Page gel? 

Appreciate any further information that will help me understand how these 
components of the Western blot work. 

Re: How do Western blots work?

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