MadSci Network: Biochemistry |
Hi Priya,
I hope I understand you right: you took these potatoes out of the ground
and measured the catalytic activity on the same day, or at least after a
short period for the first time - and then after 3, 6, ... days
again.
If that is right, the explanation is quite a simple one, and one that can
be applied to nearly every reaction with sensitive substances.
If you just put a chemical substance (i.e. actually everything) on the
table and wait, you will have an exponential decrease of the concentration
of the substance. Why? You have oxygen and moisture in the air, you have
UV-light if you don't protect it against light. So every substance that is
sensitive against one of those will be destroyed, bonds break (UV-light ->
the same what happens to your DNA if you are lying in the sun! :) or you
have a reaction with oxygen in the air.
For example Vitamin C, it's sensitive against moisture, oxygen and
UV-light, so if you boil your peppers or whatever, don't do it for a long
time!
Your catalytic activity varied only by a small amount because your
t1/2, the time after half of the substance is destroyed, is
quite large - if you try it with other enzymes, you will find stronger
variations.
I hope that was your question!
Bye,
Andreas
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