MadSci Network: Biochemistry

Re: can we used chloroform to denature protein?

Date: Tue Aug 8 13:13:56 2000
Posted By: Sarah Tegen, Grad student, Molecular and Cell Biology, UC-Berkeley
Area of science: Biochemistry
ID: 964674805.Bc

yes, chlorform is also hydrophobic and can denature proteins.  One thing 
that is commonly done to precipitate proteins is to make a mixture of phenol 
and chloroform and add that mix to the aqueous phase to do the 
precipitation.  The presence of chloroform in the phenol helps stabilize the 
interface between the aqueous and organic layers to make the separation 

Good luck!

Current Queue | Current Queue for Biochemistry | Biochemistry archives

Try the links in the MadSci Library for more information on Biochemistry.

MadSci Home | Information | Search | Random Knowledge Generator | MadSci Archives | Mad Library | MAD Labs | MAD FAQs | Ask a ? | Join Us! | Help Support MadSci

MadSci Network,
© 1995-2000. All rights reserved.