Re: can we used chloroform to denature protein?

yes, chlorform is also hydrophobic and can denature proteins.  One thing 
that is commonly done to precipitate proteins is to make a mixture of phenol 
and chloroform and add that mix to the aqueous phase to do the 
precipitation.  The presence of chloroform in the phenol helps stabilize the 
interface between the aqueous and organic layers to make the separation 
easier.

Good luck!
-Sarah