MadSci Network: General Biology |
it seems that the most straight forward way to approach this experiment would be to expose the organisms to different wavelengths of light and monitor growth. this would allow you to see which wavelength allows optimal growth. there is much literature on the pigments directly involved in photochemistry and light harvesting which are the very first steps in photosynthesis. there is also much literature on the wavelengths that agricultural plants use. it has been shown that blue and red light are most often used. it will also depend on the organism of interest. algae and bacteria will layer at different depths in water and may develop different pigments to ensure survival. ok....back to the experiment. you can buy full spectrum light bulbs from the store and some filters (the plastic that 3-d glasses are made from). depending on the number of different wavelengths you may need to buy the filters from an optics catalog (although this will be very expensive). this is a primitive experiment, but there are alot of things that you will need to worry about. you will have to make sure that the light each box recieves is equal and that temperature and CO2 levels are constant. you will have to run most of these in duplicate and have controls without the filters. another inconvienence is the lenght of time it will take to complete this experiment. if you are working with plants and not algae or bacteria then you may be in for the long haul. there are many other ways to run experiments like this but they involve expensive fluorimeters. if you have access to an instrument like this then contact me and i will help you with a much easier and less time consuming experiment.
Try the links in the MadSci Library for more information on General Biology.