MadSci Network: Biochemistry
Query:

Re: What tests are there for sugar other than Benedict's reagent?

Date: Fri Sep 15 16:44:15 2000
Posted By: Kevin Ramsey, Staff, Speciality Sweeteners, Chr. Hansen, Inc.
Area of science: Biochemistry
ID: 968347648.Bc
Message:

The test for sugar composition that is commonly used in the sugar industry 
is called Lane Eynon.  But unfortunately for you it tests for reducing 
sugars only (first you test for reducing sugars in the sample, then you 
invert the sucrose components in the sample to reducing sugars and test 
the entire sample for reducing sugar total.  You then take that number and 
subtract it from your original reading for reducing sugar and by 
difference we get sucrose).
The other test we sometimes use is High Performance Liquid Chromotography 
(HPLC).  There are columns, used in conjuction with RI detectors, that can 
read quantatively sugars (sucrose, maltose, maltotriose, glucose, and 
fructose).  This is probably your easiest and most accurate bet.
There are other analysis for sugars, such as the use of a polorimeter, but 
they follow the same format as Lane Eynon.



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