|MadSci Network: Botany|
Hi, In answering this question, I suppose that you have got the sap from pine trees. By using chromatography, you can quickly classify the different kinds of sugar in the sap solution. Then there are two methods (to my knowledge, there should more of them) can be used to measure the concentration of every sugar. The first one is enzyme assay. To do this you need specific enzymes that digest sugar perform the digestion, and by comparing the difference of enzyme before/after digestion, you can calculate the concentration of sugar. The second one is quite equipment-dependent: using high performance liquid chromatography (HPLC). This method will be very correct, though. Reference about HPLC: http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html
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