MadSci Network: Chemistry |
Chromatography is a name given to a whole range of techniques used to separate mixtures into their individual components. It was invented and named by a Russian botanist called Mikhail Tswett during the early years of the 20th century. The techniques use the fact that different molecules often have different affinities for 2 phases, a stationary phase and a mobile phase. The most common types of chromatography include Gas Liquid Chromatography (GC)which uses a Liquid stationary phase and a gas mobile phase and High Performance Liquid Chromatography (HPLC) which uses a liquid stationary phase and a liquid mobile phase. There are many variations on these techniques, and chromatography is probably the most widely used technique in a modern lab today, for example, most forensic analysis on blood and urine is done by either GC or HPLC. I have not gone into very much detail, it would take me all day, but I include a web site which you should find interesting, there is even an animation showing two different types of molecules traveling down a column, and how their different affinities result in them being separated. Thank you for the questions & est wishes. http://srd.yahoo.com/drst/245240/*http://ull.chemistry.uakron.edu/chemsep/
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