Re: Is there a method for biassing towards IgG in mAb production

Probably the best way to bias toward IgG production is to hyperimmunize 
subcutaneously and to use draining lymph nodes rather than spleens for 
fusion.  The first injection (50-100 microliters) should be in Complete 
Freund’s Adjuvant subcutaneously near the hind feet.  Four subsequent 
injections (50-100 ul) are given subq. in PBS.  Fuse one day after the 
last injection.  Do not use peak serum antibody titers as a guide for when 
to fuse – studies have shown that dividing cells fuse preferentially.  The 
antibody-secreting plasma cells are non-dividing.
Dissect the draining lymph nodes, which will be fairly large because of 
the CFA stimulation.