Does plasmid size affect transformation efficiency? How significant is it?

Subject: Does plasmid size affect transformation efficiency? How significant is it?

Date: Mon Nov 20 15:35:36 2000
Posted by Timothy
Grade level: 10-12 School: Bensalem High School
City: Bensalem State/Province: Pa Country: USA
Area of science: Molecular Biology
ID: 974752536.Mb

Message:


I am performing a nested deletion of base pairs of a plasmid.  The extent 
to which the plamsid is degraded is easily controlled, so this is where i 
vary my experiment.  I want to know whether this will have a significant 
impact upon the bacterium's ability to transform.

Re: Does plasmid size affect transformation efficiency? How significant is it?

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