|MadSci Network: Molecular Biology|
Dear Jani Rati, Thank you very much for your answer.I presumed that my question was not very clear to you. What I really don't understand is why an E.coli cell can not take two different recombinant plasmids( same vector but different insert DNA). For example, when you transform E.coli with mixture of partial Sua3A fragments ligated to BamHI cut pUC18, an E.coli cell might take two or more of different recombinant pUC18 since we do not have any control over this taking-up event. So, when this E.coli cell grows into a colony, the plasmid(S) extracted from it should contain more than one kind. But that's not the case as you know. The question is why not? Very confusing to me since all the rec plasmids come from the same pUC18 with the same ori and same evrything. May be it's a dumb question but do help me. Thank you very much in advance. Preecha Homchampa ID of the original question is 975327118.Mb
Re: Follow-up question to Jani Rati
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