| MadSci Network: Cell Biology |
The pigments in beetroot are called betalaines and are found in all plants
belonging to a taxonomic group known as "Caryophyllales". There they replace the
xantophylles as pigments found in the vacuole and have a color-span from yellow
to orange. In contrast to the xantophylles they do not show a pH-dependent change
of color.
The amount of pigment extracted from beetroot pieces is only indirectly dependent
on the concentration of detergent in the solution you use. Detergents help bring
fat and lipids into solution. For this reason they are used in many biochemical
methods to help break down membranes. In your experiment higher detergent
concentration leads to a more efficient membrane destruction, opening more
vacuoles and so setting free more pigment.
Information on this can be found in every biochemistry textbook ("Biochemistry",
Stryer) and most chemistry textbooks.
Try the links in the MadSci Library for more information on Cell Biology.