|MadSci Network: Molecular Biology|
I have read that there exists a DNA saturation curve for the transformation of E Coli. Depending upon how a cell is made competent, the optimal DNA concentration will change, and so will the DNA saturation curve. I was wondering why there may exist too much DNA for the cells, if it is not that every cell does indeed accept the plasmid. It seems logical that the more DNA there is the more E Coli cells would be transformed. According to resources there is an optimal DNA saturating condition, and this seems to contradict natural belief that more is better. Also, suppose one were to perfom an experiment using pAMP, pKAN, and 1:1 pAMP/pKAN solutions. Would the cells would the curve of the double transformation solution (pAMP/pKAN) be similar to the only pAMP and only pKAN solution? Or would the amount of double transformants increase dramatically at saturation levels of DNA. I hope i provided enough information for you to answer my question. Thank you.
Re: What effect would DNA concentration have on E Coli Transformation?
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