MadSci Network: Neuroscience |
Toko, The method of staining neurons with silver nitrate (AgNO3) was developed in 1873 by the Italian scientist Camillo Golgi. This method is often called the Golgi stain and also silver impregnation. A tissue sample is treated with potassium bichromate and then with silver nitrate. The silver nitrate stains the surface of some neurons causing them to appear dark black. The silver cation is reduced to neutral silver metal, which is jet black. For reasons that are not known, this method thoroughly stains about 5% of neurons while nearby neurons are completely unaffected. If all neurons were stained, one would only see a mass of black. The usefulness of this stain is its unusual selectivity. Golgi’s staining method allowed him to conduct pioneering work on the study of neuronal structure for which he won the Nobel prize in 1906.
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