Cytosine appears lighter in colour than other nucletide bases under UV?

Subject: Cytosine appears lighter in colour than other nucletide bases under UV?

Date: Sat Feb 24 10:11:32 2001
Posted by Alex
Grade level: undergrad School: University of Bath
City: Bath State/Province: Avon Country: United Kingdom
Area of science: Biochemistry
ID: 983027492.Bc

Message:


Recently did an experiment, Chromatography of Nucleic Acid Bases.  Once the DNA 
and RNA had been hydrolysed using 72% Perchloric acid (Does this help) and then 
heated, we had to spot them on Whatman paper.  Papers were run overnight and 
viewed with UV in the morning.  We had to deduce which bases were present in 
the samples.  But loooking at the samples in UV, I noticed that the spots of 
Cytosine (Rf 0.47) were considerablely lighter than the other bases.  Why is 
this...

Re: Cytosine appears lighter in colour than other nucletide bases under UV?

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