|MadSci Network: Biochemistry|
Recently did an experiment, Chromatography of Nucleic Acid Bases. Once the DNA and RNA had been hydrolysed using 72% Perchloric acid (Does this help) and then heated, we had to spot them on Whatman paper. Papers were run overnight and viewed with UV in the morning. We had to deduce which bases were present in the samples. But loooking at the samples in UV, I noticed that the spots of Cytosine (Rf 0.47) were considerablely lighter than the other bases. Why is this...
Re: Cytosine appears lighter in colour than other nucletide bases under UV?
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