MadSci Network: Molecular Biology

Subject: what does Oligo (dT)-cellulose chromatography mean?

Date: Fri Jun 29 11:25:54 2001
Posted by Tyron
Grade level: grad (science) School: FSU
City: Tallahassee State/Province: FL Country: USA
Area of science: Molecular Biology
ID: 993828354.Mb

In the protocol of an RNA isolation, I read that: "Poly(A)+ RNA was isolated 
from total RNA using oligo(dT)-cellulose chromatography, and was wondering what 
Poly(A)+ means as well as oligo(dT) cellulose chromatography. The paper deals 
with isolation of cellulase cDNAs from a microorganism.

Re: what does Oligo (dT)-cellulose chromatography mean?

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