How do you identify pigments as they show up on paper chromatography?

Subject: How do you identify pigments as they show up on paper chromatography?

Date: Fri Dec 14 00:02:18 2001
Posted by Dina Wang
Grade level: 10-12 School: Sir John A. Macdonald C.I.
City: Toronto State/Province: Ontario Country: CANADA
Area of science: Botany
ID: 1008306138.Bt

Message:


  I did a chromatography of geranium leaves with acetone and petroleum ether
(1:9) solvent.  I ended up with about 7 bands of colour visible under white 
light and two that were only visible under UV light.  How do you determine what 
the names of the different pigments are?  I've tried calculating the Rf values, 
but they just don't seem to match up with any values I've found over the 
internet!  Any help would be appreciated!  Thanks.

Re: How do you identify pigments as they show up on paper chromatography?

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