MadSci Network: Cell Biology |
Embryonic stem (ES) cells are the most controversial cell type derived from humans and have also been derived from a range of other animals, including primates. However, it is unlikely that ES cells derived from primates will be useful in humans since the human immune system will see the primate cells as foreign and destroy them. There are ways of getting round this problem, for example, cloning a patient's cells to enable isolation of ES cells identical to them. However, this technique will be very costly and is still controversial as human embryos are used for the cloning procedure. It has been proposed that bovine embryos be used as a surrogate embryo to isolate human ES cells. In this method, a human nucleus is placed into an enucleated bovine oocyte and cultured in vitro to blastocyst stage to isolate ES cells from the inner cell mass/epiblast. Contaminating bovine mitochondria could be inactivated before the human nucleus is inserted and human mitochondria could then be added. This technique, however, is contoversial as it is a form of xenotranplantation. (See Ward CM (2002). The isolation, culture and therapeutic application of pluripotent stem cells derived from human embryos. Expert Opinion on Therapeutic Patents. 12 (9):1395-1402) The most likely method to be focused on in the next few years is the dedifferentiation of somatic cells from patients to produce a pluripotent cell type capable of differentiating into a range of cell lineages. As these "stem cells" will be identical to the patient their immune system will not see the cells as foreign. I hope this has answered your question.
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