|MadSci Network: Cell Biology|
Thiamine or Vitamin B1 is an important cofactor in the Krebs or citric acid cycle which takes place in the mitochondria. Before thiamine is incorporated into the enzymes pyruvate dehydrogenase and alpha- ketogluterate dehydrogenase, the pyrophosphate group is added to thiamine to make thiamine pyrophosphate. It is this molecule that participates along with other cofactors in the catalytic oxidative decarboxylation of pyruvate (a product of glycolysis) to form acetyl CoA. Acetyl CoA then enters the citric acid cycle and ultimately produces NADH and FADH2 which then participate in the electon transport chain. Stryer, Lubert, Biochemistry, 3rd edition, 1988, Chapter 16
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