MadSci Network: Genetics |
Dear Esperanza,
The technique that you describe is often referred
to as
"ABO genotyping", in contrast to the typical serotyping method
that
you probably read about, which only determines the ABO phenotype. It
is a
relatively new procedure that has not yet been standardized, as serotyping
has,
so a variety of different techniques appear to be currently in use.
However, the method in most widespread use to date seems to be based on
that
developed by the General Hospital & University Clinics at Innsbruck,
Austria
in 1996 using the polymerase chain reaction with sequence-specific primers
(PCR-SSP).
It is summarized in this abstract
from the article published by Gassner et al. in the journal, Blood,
&
described in more detail by the complete manuscript, ABO
Glycosyltransferase Genotyping by Polymerase Chain Reaction Using
Sequence-Specific Primers (in PDF format requiring the free Adobe
Acrobat Reader to view). Because this is such a new procedure,
I'm not
sure how many commercial labs are currently offering it & did not come
across
any during my search. However, if you are interested in pursuing this
further, a more exhaustive search using the term, "ABO
genotyping",
might locate such a lab for you. I will caution you that such a test
is
likely to be fairly expensive compared to the much simpler ABO serotyping,
so
you might want to try & locate a university research lab that's already
working with ABO genotyping & may be willing to test you without
charge.
Thanks for the interesting question,
Jeff Buzby, Ph.D.
CHOC Research Institute
MadSci Genetics Network
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