MadSci Network: Molecular Biology

Subject: Degradation of linearized DNA in E.coli

Date: Wed May 14 11:32:39 2003
Posted by Jonathan
Grade level: grad (science) School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Molecular Biology
ID: 1052929959.Mb

How fast and efficient is linearized DNA degraded in E.coli? 
What happens when electroporating E.coli with linearized DNA containing 
selection marker and origin of replication? 
Does it get lost or does it linger around?
Thanks a lot for helping out! Jo

Re: Degradation of linearized DNA in E.coli

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