MadSci Network: Biochemistry

Subject: Prestaining Proteins for Isoelectric Focusing

Date: Sun Oct 26 15:16:11 2003
Posted by No name entered.
Grade level: undergrad School: No school entered.
City: No city entered. State/Province: No state entered. Country: No country entered.
Area of science: Biochemistry
ID: 1067202971.Bc


I am developing a microfluidic chip for two dimensional separation of 
proteins, using IEF as my first separation step. What can I do to 
monitor the migration of proteins in this step. Are there any 
prestains I can use on the proteins without denaturing them or 
changing their PI points? Are there naturally colored protein 
standards I can use (I have used BioRad's Protein Standards which are 
supposed to be naturally colored, and I can only see one very very 


Re: Prestaining Proteins for Isoelectric Focusing

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