|MadSci Network: Biochemistry|
Hi! I am developing a microfluidic chip for two dimensional separation of proteins, using IEF as my first separation step. What can I do to monitor the migration of proteins in this step. Are there any prestains I can use on the proteins without denaturing them or changing their PI points? Are there naturally colored protein standards I can use (I have used BioRad's Protein Standards which are supposed to be naturally colored, and I can only see one very very faintly) Thanks
Re: Prestaining Proteins for Isoelectric Focusing
Try the links in the MadSci Library for more information on Biochemistry.