Subject: Prestaining Proteins for Isoelectric Focusing

Hi!

I am developing a microfluidic chip for two dimensional separation of 
proteins, using IEF as my first separation step. What can I do to 
monitor the migration of proteins in this step. Are there any 
prestains I can use on the proteins without denaturing them or 
changing their PI points? Are there naturally colored protein 
standards I can use (I have used BioRad's Protein Standards which are 
supposed to be naturally colored, and I can only see one very very 
faintly)

Thanks

Re: Prestaining Proteins for Isoelectric Focusing