MadSci Network: Biochemistry

Subject: Can bacteria express proteins with disufide bonds?

Date: Mon Nov 17 16:07:50 2003
Posted by Jimmy
Grade level: undergrad School: California State University, Fullerton
City: CA State/Province: 92834 Country: USA
Area of science: Biochemistry
ID: 1069106870.Bc

I am aware that insulin can be expressed in E. coli, but it's not until 
chemical treatment of the peptides that oxidizes the cys residues to make the 
disulfide bridge.  But I'm unclear as to whether or not the cytoplasmic 
environment of bacteria is suitible to maintain/express proteins with disulfide 
bonds.  Thanks for your time

Re: Can bacteria express proteins with disufide bonds?

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