MadSci Network: Cell Biology |
You, as an undergraduate, should realise that, since it is a 'theory' and not a 'law', your question CANNOT have a simple answer. The purpose of your education is to train you to analyse facts and draw your own conclusion. So... what is the theory and what are the facts? The theory suggests that chloroplasts and mitochondria began as free- living organisms; that they became trapped by the earliest eukaryotic cells and that they then evolved together to become totally interdependent. The evidence for this is considerable - and, always a good sign, the more we learn about the detailed biochemistry of the cell, the more it supports the theory. Prokaryotes invariably have 70S ribosomes; eukaryotes 80S. Both of these organelles have the prokaryotic form. Their rate of mutation being known, studies have suggested that the origin of these organelles roughly coincided with the changes in the Earth's atmosphere and fossil record suggesting that life had 'moved up a gear'. Oxygen being poisonous, the early cells probably benefitted more from the lowered oxygen tension than from the release of energy in the form of ATP - that came later. Both organelles have closed circular coiled DNA - the form found in prokaryotes. If these organelles were part of the usual pattern of cell organelles, synthesised in response to nuclear DNA, then it is unclear why extra-nuclear DNA would be required. Furthermore, the organelle DNA codes for the proteins at the heart of the organelle - the inner membrane proteins and those of the Kreb's cycle and ox-phos pathway (mitos)and those of the light and light-independent reactions of photosynthesis (chloroplasts). In the case of mitos, this is about 50% of the total; in chloroplasts about 70%. Both organelles have two membranes (some suggest the thylakoid membranes of chloroplasts are a third layer, but they are normally contiguous with the inner membrane, so I suspect not). The outer membranes are synthesised by the host cell; the inner membrane substantially by the organelle DNA. This is consistent with the idea that they were originally 'engulfed' by the host cell - as many Protists do to their prey today. Its influence on cell biology is that it suggests a sequence for the evolution of life on Earth at a time when the fossil record is necessarily all but non-existent. We have an insight into life during the first 25% of the development of modern life-forms. It suggests that life continually adapts itself to exploit prevailing conditions and that co-operation is sometimes of benefit to both species - even if one eventually becomes dependent on the other. Both organelles have a 'semi-autonomous' existence in which their numbers in any one cell appears to be outside the direct control of the nucleus. This means that they can be affected by certain antibiotics (such as chloramphenicol)which have no effect on the rest of the cell - they can be selectively 'poisoned'. It is to be hoped that the human genome project will enable us to see the genes that are influential in the regulation of these organelles and enable us to gain some insight into their origin. Perhaps life is not, always, 'red in tooth and claw'! Like all theories, it originated from Man's continual search to find 'the ultimate truth'. In other words, scientists wanted an explanation for some unusual and apparently contardictory facts and the endosymbiont theory was devised as a possible explanation. I am unaware of any serious evidence contradicting it - this has been 'mainstream' theory for well over 30 years.
Try the links in the MadSci Library for more information on Cell Biology.