Re: How does met-enkephalin synthesised?

Date: Sat Apr 3 16:34:58 2004
Posted By: Siddharth Srivastava, Undergraduate, Biochemistry, Columbia University
Area of science: Biochemistry
ID: 1080470312.Bc

Message:


Met-enkephalin is synthesized enzymatically in a sequential manner. 
Carboxypeptidase Y is used in the stepwise synthesis of met-enkephalin.

Ac-Tyr-OEt => Ac-Tyr-Gly-NH2 with Carboxypeptidase Y, Gly-NH2, pH 9.0

Ac-Tyr-Gly-NH2 undergoes deamidation to produce Ac-Tyr-Gly-OH

Ac-Tyr-Gly-OH => Ac-Tyr-Gly-OEt with ethanol/HCl

The process is then repeated with Carboxypeptidase Y, (an amino acid)-NH2, 
pH 9.0 until the necessary amino acids have been added, to produce met-
enkephalin:

Ac-Tyr-Gly-OEt => Ac-Tyr-Gly-Gly-Phe-Met-OH with Carboxypeptidase Y, (AA-
NH2), pH 9.0

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