|MadSci Network: Biochemistry|
There was no single experiment or even a short series of experiments that led to the formulation of the Krebs/citric acid cycle. It was a lot of laboratories over many years that led to Krebs' 1937 paper first proposing the cycle. Krebs certainly contributed experimental work in addition, but so did many other labs over many years. Krebs contribution was in significant part the recognition that the data suggested a catalytic cycle in which only small amounts of the substrates were needed since the intermediates could be regenerated. That is, it was a circle or a cycle of reactions. The following link give the biography of Sir Hans Krebs that is posted on the Nobel Foundation's website. On that page is a link to a pdf file of Krebs' Nobel lecture. It's as good a history of the discovery as I know of. In fact, Krebs clearly downplays his own role and credits many other people with various discoveries. http:// nobelprize.org/medicine/laureates/1953/krebs-bio.html The following are links to animations and/or explanations of the Krebs/citric acid cycle. http://www.johnkyrk.com/krebs.html http://www.science.smith.edu/departments/Biology/Bio231/krebs.html
Try the links in the MadSci Library for more information on Biochemistry.