MadSci Network: Chemistry
Query:

Re: what specific procedure can be done to test for amount of sugar in candy?

Date: Fri Feb 25 13:58:39 2005
Posted By: Kevin Ramsey, Staff, Speciality Sweeteners, Chr. Hansen, Inc.
Area of science: Chemistry
ID: 1107316931.Ch
Message:

I believe the tests you can utilize is either polarization or Lane and 
Eynon.  
Polarization - Polarized Light: Ordinary light such as sunlight is an 
electromagnetic wave, which vibrates in an infinite number of planes.  
Some optical devices cause the light beam to vibrate in only one plane.  
This light is then said to be polarized.  Sugars have the power to rotate 
the plane of polarization.  And this angle which the plane of polarization 
is rotated can be measured.  A Saccharimeter or polarimeter are such 
instruments.  The easiest method I found would be in the International 
Commission for Uniform Methods of Sugar Analysis (ICUMSA).

Lane and Eynon:  This method utilizes the concept that Reducing Sugars 
(glucose and fructose) have the ability to reduce copper in the cupric 
state to cuprous oxide.  Under specified conditions, the amount of copper 
that is reduced is in proportion to the quantity of the reducing sugars 
present.  This method is a titration which allows the scientist to observe 
a color change when all of the copper has been reduced.
The ICUMSA book also has a procedure for the determination of sugar via 
the Lane and Eynon method.



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