|MadSci Network: Biochemistry|
I am trying to find out what happens to the proteins in the bovine serum we use as a lubricant in testing hip implants. I am taking samples of the serum at various time intervals and I'm testing it for protein content with capillary electrophoresis and with SDS-Page and Western Blot. I need to remove any denatured proteins to analyse what is left. Because they tend to precipitate, I think centrifuging the samples will remove the denatured proteins, but I have no idea how long I should centrifuge so I can get them all but not cause more denaturing to occur.
Re: How long do I centrifuge serum to remove denatured proteins?
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