MadSci Network: Biochemistry

Subject: How long do I centrifuge serum to remove denatured proteins?

Date: Mon Apr 18 15:25:49 2005
Posted by Sharon
Grade level: grad (science) School: Loma Linda Univ. Medical Center
City: Loma Linda State/Province: CA Country: USA
Area of science: Biochemistry
ID: 1113855949.Bc

I am trying to find out what happens to the proteins in the bovine serum we 
use as a lubricant in testing hip implants.  I am taking samples of the serum 
at various time intervals and I'm testing it for protein content with 
capillary electrophoresis and with SDS-Page and Western Blot.  I need to 
remove any denatured proteins to analyse what is left. Because they tend to 
precipitate, I think centrifuging the samples will remove the denatured 
proteins, but I have no idea how long I should centrifuge so I can get them 
all but not cause more denaturing to occur. 

Re: How long do I centrifuge serum to remove denatured proteins?

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