|MadSci Network: Molecular Biology|
Sorry there is not enough room in the box above. Do protein have problems sometimes when they derived from mRNA, made into DNA, spliced into a plasmid and expressed (cloning)? When I have expressed proteins in this manner, they have aggregated. I was wondering if the lack of introns may cause this problem. Could introns have some function or activate some enzyme that enhances say protein folding, for example? Are introns really useless?
Re: When expressing a protein derived from cDNA, can lack of introns cause prob
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