MadSci Network: Molecular Biology
Query:

Re: what is unit for DNA?

Date: Mon Sep 5 22:23:13 2005
Posted By: tarun gupta, Undergraduate, bioinformatics, ggdsd college of post graduation
Area of science: Molecular Biology
ID: 1125049069.Mb
Message:

Hi Akhunzada,
               Generaly we measure DNA in Angstroms. Like each base pair 
is 3.4 angstroms apart. But here are some detailed Units:



1 mg = 10-3 g.
1 ug = 10-6 g.
1 ng = 10-9 g.
1 pg = 10-12 g.

1 kb of DNA	= 6.5 x10+5 Daltons of duplex DNA (sodium salt)
	= 3.3 x10+5 Daltons of single-stranded DNA (sodium salt)
	= 3.4 x10+5 Daltons of single-stranded RNA (sodium salt)

Average MW of a deoxynucleotide base = 324.5 Daltons
Average MW of a deoxynucleotide base pair = 649 Daltons

1 ug/ml of DNA = 3.08 uM phosphate
1 ug/ml of 1 kb of DNA = 3.08 nM 5' ends
1 mol of pBR322 = 2.83 x10+6 g.
1 pmol of linear pBR322, 5' ends = 1.4 ug
1 A260 unit of duplex DNA = 50 ug
1 A260 unit of single-stranded DNA = 37 ug
1 A260 unit of single-stranded RNA = 50 ug

1 kb of DNA	= 333 amino acids of coding capacity
	= 37,000 daltons

Densities (50% GC):

		RF I (supercoiled)  ds DNA	1.709 g/ml
		RF II (nicked)	ds DNA	1.54 g/ml
		ss DNA			1.726 g/ml
		ss RNA			1.90 g/ml
		protein			1.33 g/ml





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