|MadSci Network: Physics|
There is no one answer to your question. Pseudo-confocal microscopy is basically any attempt to cheat out of doing the real thing. Confocal microscopy uses both eyes in order to give you some depth perception on the slide itself. This is advantageous for the user to get a better idea of edges, thickness, and overlaps without dying the specimen. So there are a couple of methods to do this, the one I know most about is DIC, Diffferential Interference Contrast, which uses two prisms to refract the light at varying wavelengths to 'pop' the edges. This website gives you the basics on a couple of other methods. Good luck! http://www.mc.vanderbilt.edu
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