Re: is it possible to culture the T lymphocyte

Dear Amol,
The answer is yes. Lymphocytes have been cultured for more than 30 years
now. These cells have a important capability of proliferation, which is one
of the basis of the immunological theory. In last years with a better
understanding of the stem cell biology, this notion that differentiated
cells are unable to replicate has losen its strenght. Foor example, even in
the brain, which the cells, the neurons, were thought not to proliferate
after embrionary period. Today we know that certain cells, known as
neuronal progenitors, are capable of replication and renewal of neuron
populations under certain situations. So, cells are much more flexible than
we thought before.
If I understood right, your second question is: Which cells are infected by
HIV besides T CD4 lymphocytes?
HIV attaches himself to a complex of molecules in membranes, whose the main
one is the CD4 molecule. This molecule can be found in monocytes,
macrophages and endothelial cells. Therefore, the cells also can  be
infected by HIV.
Hope all is clear and never hesitate in asking. Best regards,
Vitor