in spectrofluorimetry, what is the purpose of having an emission wavelength

Subject: in spectrofluorimetry, what is the purpose of having an emission wavelength

Date: Sat Mar 25 15:19:52 2006
Posted by No name entered.
Grade level: 10-12 School: No school entered.
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Area of science: Biochemistry
ID: 1143325192.Bc

Message:


Hi, I am trying to measure caspase activity using spectrofluorimetry which is 
based on the paper "The Adapter Protein Apoptotic Protease-activating Factor-1 
(Apaf-1) is proteolytically Processed during Apoptosis". However, I am 
unfamiliar with this device and few websites provide sufficient information 
regarding this topic. Is fluorescence intensity the same as the emission 
wavelength since fluorescence is the light emitted by the excitation 
wavelength? If not, what is the purpose of having an emission wavelength if 
thats what you're measuring? 

Your help is GREATLY appreciated.
Thankyou for your time.

Re: in spectrofluorimetry, what is the purpose of having an emission wavelength

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