|MadSci Network: Biochemistry|
Hi, I am trying to measure caspase activity using spectrofluorimetry which is based on the paper "The Adapter Protein Apoptotic Protease-activating Factor-1 (Apaf-1) is proteolytically Processed during Apoptosis". However, I am unfamiliar with this device and few websites provide sufficient information regarding this topic. Is fluorescence intensity the same as the emission wavelength since fluorescence is the light emitted by the excitation wavelength? If not, what is the purpose of having an emission wavelength if thats what you're measuring? Your help is GREATLY appreciated. Thankyou for your time.
Re: in spectrofluorimetry, what is the purpose of having an emission wavelength
Try the links in the MadSci Library for more information on Biochemistry.