| MadSci Network: Biochemistry |
Based on the literature references below, it looks like there are good published methods for TLC separation. For reverse-phase chromatography, the common solvent system of acetonitrile/water is used. For normal-phase chromatography, three systems used are toluene/acetone, chloroform/2- propanol and ethyl acetate/hexane. Visualization can be accomplished at 365 or 254 nm due to the benzyl group chromophores. Iodine vapors will can also be used to aid visualization as well.
I hope this helps.
Details are available in these references:
Simultaneous analysis of beauvericin and moniliformin in fungal cultures and in cereal grain samples. Kostecki, M.; Grabarkiewicz-Szczesna, J.; Golinski, P. Dep. Chemistry, August Cieszkowski Agricultural University, Poznan, Pol. Mycotoxin Research (1997), 13(1), 17-22.
A Rapid Method for the Determination of the Fusarium Mycotoxin Beauvericin in Maize, Fresenius. Josephs, R.D., Krska, R., Schuhmacher, R., Grasserbauer, M., J. of Anal. Chem. 363, 130-131 (1999)
Production of the Mycotoxins Fusaproliferin and Beauvericin by South African Isolates in the Fusarium Section Liseola. Shephard, G.S., Sewram, V., Nieuwoudt, T.W., Marasas, W.F.O., Ritieni, A., J.Agric. Food Chem., 47, 5111-5115 (1999)
Natural Occurrence of Beauvericin in Preharvest Fusarium subglutinans Infected Corn Ears in Poland. Logrieco, A., Moretti, A., Ritieni, A., Chelkowski, J., Altomare, C., Bottalico, A., Randazzo, G., J. Agric. Food Chem., 41, 2149-2152 (1993)
Occurrence of Fusaproliferin, Fumonisin B1 and Beauvericin in Maize from Italy. Ritieni, A., Moretti, A., Logrieco, A., Bottalico, A., Randazzo, G., Monti, S.M., Ferracane, R., Fogliano, V., J. Agric. Food Chem., 45, 4011-4016 (1997)
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