|MadSci Network: Cell Biology|
Hello, Usually, the solvent of choice is DMSO (DiMethylSulfOxide). Up to 1 or 2 % there should be no visual effect on the cells, but there might be a subcellular effect, especially for the markers you want to measure. I have never tested diethyl ether on cells, but I expect it should have the same effect on membranes as chloroform. Methane ? You mean methanol, probably. Alcohols will have the worst effect on your cells, dont go more than 1/1000. Thus, I would say : - Check at the company where the chemicals come from, or on the data sheet. There should be a chemist able to tell you in which solvent you have the maximum solubility. - Once you have this, extrapolate the final concentration of your solvent in your culture. Try to minimize it. For example, if you can make 2mM in DMSO, and the highest concentration in your study is 10uM, test a 0.5% DMSO culture, and make a time-curve of your markers expression of the DMSO versus non-DMSO. If you have no effect, it's perfect. Try to check in your bibliography. You are probably not the very first to use this chemical. Other tips I can give you are : DMF (DiMethylFormamide) is more stinky but solubilizes as well as DMSO For really non-cooperative chemicals, an hour sonication should do the job... Hope it helps... Good luck... S.
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