|MadSci Network: Molecular Biology|
Hi Jodie, It's difficult to say exactly why calf thymus DNA was used in the article you're reading without knowing what the specific experiment is. In general, calf thymus DNA is used for a number of purposes, including (1) as a substrate for DNA-modifying enzymes, (2) in DNA-binding assays, (3) as carrier DNA, and (4) as a blocking agent in hybridization solutions. I would guess that calf thymus DNA was used as a non-specific control for human DNA in your paper. Why calf thymus DNA? Probably because it is readily available in large quantities, and not because of any particular similarity to human DNA. Hope this helps, and good luck with your article review. Joy
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